human derived glioma cells  (Crown Bioscience)

Journal: Advanced Functional Materials

Article Title: MCL1 Inhibitor Augmented the Anti‐Glioma Efficacy of Paclitaxel Utilizing a Multifunctional Cascade Nanodrug System

doi: 10.1002/adfm.202414499

Figure Lengend Snippet: Figure 2. Permeability of the BBB and glioma targeting by the RVG29 and FA-modified nanodelivery system. a) Construction of the BBB model in vitro and schematic diagram of nanoparticle infiltration of BBB. b) Cumulative BBB penetration ratio between Ce6@NPs and Ce6@RVG-NPs at 1, 2, 4, 6, 8, and 12 h (n = 3). c) Flow cytometry analysis to detect nanoparticle uptake in GL261 cells after 4 h treatment with Ce6@Free, Ce6@NPs Ce6@FA- NPs and Ce6@RF-NPs. d) Quantification of mean fluorescence intensity (MFI) of GL261 cell uptake (n = 3). The uptake of nanoparticles by LN229 was

Article Snippet: Cell Culture and Animals: Mouse-derived glioma cell line GL261, human-derived glioma cell lines LN229, and mouse brain microvascular endothelial cells bEnd.3 were procured from the American Type Culture Collection (ATCC, USA).

Techniques: Permeability, In Vitro, Flow Cytometry

Journal: Advanced Functional Materials

Article Title: MCL1 Inhibitor Augmented the Anti‐Glioma Efficacy of Paclitaxel Utilizing a Multifunctional Cascade Nanodrug System

doi: 10.1002/adfm.202414499

Figure Lengend Snippet: Figure 6. The activity of A12-PTX@RF-NPs against human-derived gliomas was subjected to in vivo evaluation. a) Schematic diagram of the experimental plan for the LN229-Luc cell in situ transplantation tumor model. b) Body weight change curve of in situ tumor mice (N = 3). c) In vivo luminescence images of orthotopic glioma. d) Quantitative analysis of luminescence intensity in gliomas in situ on the fifteenth day. (N = 3). e) Survival curve of mice (N = 6). Data are presented as mean ± SEM and analyzed by one-way analysis of variance. *p < 0.05 and **p < 0.01.

Article Snippet: Cell Culture and Animals: Mouse-derived glioma cell line GL261, human-derived glioma cell lines LN229, and mouse brain microvascular endothelial cells bEnd.3 were procured from the American Type Culture Collection (ATCC, USA).

Techniques: Activity Assay, Derivative Assay, In Vivo, In Situ, Transplantation Assay, Quantitative Luminescence

Journal: PLOS ONE

Article Title: Extracellular mixed histones are neurotoxic and modulate select neuroimmune responses of glial cells

doi: 10.1371/journal.pone.0298748

Figure Lengend Snippet: Extracellular MH downregulate LPS-stimulated U118 MG human astrocytic cell mediated toxicity towards SH-SY5Y human neuron-like cells and are not cytotoxic towards U118 MG cells.

Article Snippet: The glioma-derived U118 MG human astrocytic cell line was purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques:

Journal: Biomedical Optics Express

Article Title: Cytotoxicity analysis of oxazine 4-perchlorate fluorescence nerve potential clinical biomarker for guided surgery

doi: 10.1364/BOE.447773

Figure Lengend Snippet: Cytotoxicity assay results, expressed as percentage of viability of the U87 cells in dark and after 15 minutes of 10 mW/cm2 irradiation with white light (A) and λ=630 nm light (B). Cells were treated with different amounts of O4P (0, 50, 100, 200 and 400 μM) for 4 h. These data represent the relative (in percentage) cell viability with respect to the negative control after 4 independent experiments. The results were analyzed using a two-factor ANOVA test with 6 samples per group. Statistical significance is indicated by *p<0.05, **p<0.01, ***p<0.001.

Article Snippet: Cell culture and fluorophore Human-derived glioma U87 cell line (ATCC HBT-14) was grown as monolayer in Dulbeccós modified Eaglés medium (DMEM) (Lonza; Walkersville, MD, USA), supplemented with 10% fetal bovine serum (FBS) (HyClone, Global Life Science; Pasching, Austria), penicillin (100 U/mL) and streptomycin (100 μg/mL) (Lonza; Verviers, Belgium).

Techniques: Cytotoxicity Assay, Irradiation, Negative Control

Journal: Biomedical Optics Express

Article Title: Cytotoxicity analysis of oxazine 4-perchlorate fluorescence nerve potential clinical biomarker for guided surgery

doi: 10.1364/BOE.447773

Figure Lengend Snippet: Cytotoxic effect of the tested irradiations in combination with different concentrations of O4P on the percentage of cellular death in U87 cells. Results of 4 independent experiments expressed as relative Mean ± SD with respect to dark control.

Article Snippet: Cell culture and fluorophore Human-derived glioma U87 cell line (ATCC HBT-14) was grown as monolayer in Dulbeccós modified Eaglés medium (DMEM) (Lonza; Walkersville, MD, USA), supplemented with 10% fetal bovine serum (FBS) (HyClone, Global Life Science; Pasching, Austria), penicillin (100 U/mL) and streptomycin (100 μg/mL) (Lonza; Verviers, Belgium).

Techniques: Control, Concentration Assay

Journal: Biomedical Optics Express

Article Title: Cytotoxicity analysis of oxazine 4-perchlorate fluorescence nerve potential clinical biomarker for guided surgery

doi: 10.1364/BOE.447773

Figure Lengend Snippet: Representative examples of confocal microscopy images from dark control (A–H), white light (I–P) or at 630 nm irradiated cells (Q-X). Comparison between cells in the absence of fluorophore (controls A-D, I-L and Q-T) and cells treated with 100 µM of O4P. DAPI (blue), ϒH2AX (green), and PI (red). Scale bar = 50 µm.

Article Snippet: Cell culture and fluorophore Human-derived glioma U87 cell line (ATCC HBT-14) was grown as monolayer in Dulbeccós modified Eaglés medium (DMEM) (Lonza; Walkersville, MD, USA), supplemented with 10% fetal bovine serum (FBS) (HyClone, Global Life Science; Pasching, Austria), penicillin (100 U/mL) and streptomycin (100 μg/mL) (Lonza; Verviers, Belgium).

Techniques: Confocal Microscopy, Control, Irradiation, Comparison

Journal: Biomedical Optics Express

Article Title: Cytotoxicity analysis of oxazine 4-perchlorate fluorescence nerve potential clinical biomarker for guided surgery

doi: 10.1364/BOE.447773

Figure Lengend Snippet: O4P distribution (DAPI in blue, O4P in red). A) U87 confocal microscopy images treated with 50 and 100 µM of O4P either in dark or after irradiation at 630 nm for 15 and 30 min. Representative images of the localization of the fluorophore were taken after 4 hours of incubation. Nuclei labelled with DAPI are shown in blue. Scale bar = 25 μm. B) Higher magnification of merged images with no fluorophore and 100 μM. Scale bar = 20 μm.

Article Snippet: Cell culture and fluorophore Human-derived glioma U87 cell line (ATCC HBT-14) was grown as monolayer in Dulbeccós modified Eaglés medium (DMEM) (Lonza; Walkersville, MD, USA), supplemented with 10% fetal bovine serum (FBS) (HyClone, Global Life Science; Pasching, Austria), penicillin (100 U/mL) and streptomycin (100 μg/mL) (Lonza; Verviers, Belgium).

Techniques: Confocal Microscopy, Irradiation, Incubation

Journal: Biomedical Optics Express

Article Title: Cytotoxicity analysis of oxazine 4-perchlorate fluorescence nerve potential clinical biomarker for guided surgery

doi: 10.1364/BOE.447773

Figure Lengend Snippet: Cytotoxicity assay results, expressed as percentage of viability of the U87 cells in dark and after 15 minutes of 10 mW/cm2 irradiation with white light (A) and λ=630 nm light (B). Cells were treated with different amounts of O4P (0, 50, 100, 200 and 400 μM) for 4 h. These data represent the relative (in percentage) cell viability with respect to the negative control after 4 independent experiments. The results were analyzed using a two-factor ANOVA test with 6 samples per group. Statistical significance is indicated by *p<0.05, **p<0.01, ***p<0.001.

Article Snippet: Human-derived glioma U87 cell line (ATCC HBT-14) was grown as monolayer in Dulbeccós modified Eaglés medium (DMEM) (Lonza; Walkersville, MD, USA), supplemented with 10% fetal bovine serum (FBS) (HyClone, Global Life Science; Pasching, Austria), penicillin (100 U/mL) and streptomycin (100 μg/mL) (Lonza; Verviers, Belgium).

Techniques: Cytotoxicity Assay, Irradiation, Negative Control

Journal: Biomedical Optics Express

Article Title: Cytotoxicity analysis of oxazine 4-perchlorate fluorescence nerve potential clinical biomarker for guided surgery

doi: 10.1364/BOE.447773

Figure Lengend Snippet: Cytotoxic effect of the tested irradiations in combination with different concentrations of O4P on the percentage of cellular death in U87 cells. Results of 4 independent experiments expressed as relative Mean ± SD with respect to dark control.

Article Snippet: Human-derived glioma U87 cell line (ATCC HBT-14) was grown as monolayer in Dulbeccós modified Eaglés medium (DMEM) (Lonza; Walkersville, MD, USA), supplemented with 10% fetal bovine serum (FBS) (HyClone, Global Life Science; Pasching, Austria), penicillin (100 U/mL) and streptomycin (100 μg/mL) (Lonza; Verviers, Belgium).

Techniques: Control, Concentration Assay

Journal: Biomedical Optics Express

Article Title: Cytotoxicity analysis of oxazine 4-perchlorate fluorescence nerve potential clinical biomarker for guided surgery

doi: 10.1364/BOE.447773

Figure Lengend Snippet: O4P distribution (DAPI in blue, O4P in red). A) U87 confocal microscopy images treated with 50 and 100 µM of O4P either in dark or after irradiation at 630 nm for 15 and 30 min. Representative images of the localization of the fluorophore were taken after 4 hours of incubation. Nuclei labelled with DAPI are shown in blue. Scale bar = 25 μm. B) Higher magnification of merged images with no fluorophore and 100 μM. Scale bar = 20 μm.

Article Snippet: Human-derived glioma U87 cell line (ATCC HBT-14) was grown as monolayer in Dulbeccós modified Eaglés medium (DMEM) (Lonza; Walkersville, MD, USA), supplemented with 10% fetal bovine serum (FBS) (HyClone, Global Life Science; Pasching, Austria), penicillin (100 U/mL) and streptomycin (100 μg/mL) (Lonza; Verviers, Belgium).

Techniques: Confocal Microscopy, Irradiation, Incubation